dose-response—inhibition curve software Search Results


dose response curve with acitretin  (Selleck Chemicals)
92
Selleck Chemicals dose response curve with acitretin
<t>Acitretin</t> mitigates CEP bone phenotype in zebrafish. ( A ) 6dpf larvae were injected with uro-I and transferred to medium containing acitretin or DMSO. At 7dpf larvae were injected with calcein and imaged by confocal microscopy. Quantification of porphyrin fluorescence ( B ), porphyrin excretion ( C ) and operculum volume ( D ) from experiment in ( A ). Symbols represent individual larvae (12–25/group) from 3–4 independent experiments. ( E ) 6dpf larvae were injected with uro-I. At 7dpf they were transferred to medium containing acitretin or DMSO. At 8dpf larvae were injected with calcein and imaged by confocal microscopy. Quantification of porphyrin fluorescence ( F ), porphyrin excretion ( G ) and operculum volume ( H ) from experiment in ( E ). Arrowhead-operculum; box-vertebrae ( A , E ). Symbols represent individual larvae (18–64/group) from 3–4 independent experiments. ( I-K ) Larvae were treated as in ( A ) with the indicated retinoid or DMSO and porphyrin fluorescence ( I ), porphyrin excretion ( J ) and operculum volume ( K ) were assayed. Bone volume was normalized to DMSO-treated larvae set to 100%, ( D , H , K ). Porphyrin excretion was normalized to DMSO-treated larvae set to 100%, ( C , G , J ). Symbols represent individual larvae (7–44/group) from 2–4 independent experiments. Scale bars: 200 µm. Three-dimensional image reconstruction ( A , E ) was performed using Imaris 3D software v7.7 ( http://imaris.oxinst.com/ ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Dose Response Curve With Acitretin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dose-response%E2%80%94inhibition+curve+software/Acitretin/pmc08100164-184-1-5
Average 92 stars, based on 1 article reviews
dose response curve with acitretin - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier

Image Search Results


Acitretin mitigates CEP bone phenotype in zebrafish. ( A ) 6dpf larvae were injected with uro-I and transferred to medium containing acitretin or DMSO. At 7dpf larvae were injected with calcein and imaged by confocal microscopy. Quantification of porphyrin fluorescence ( B ), porphyrin excretion ( C ) and operculum volume ( D ) from experiment in ( A ). Symbols represent individual larvae (12–25/group) from 3–4 independent experiments. ( E ) 6dpf larvae were injected with uro-I. At 7dpf they were transferred to medium containing acitretin or DMSO. At 8dpf larvae were injected with calcein and imaged by confocal microscopy. Quantification of porphyrin fluorescence ( F ), porphyrin excretion ( G ) and operculum volume ( H ) from experiment in ( E ). Arrowhead-operculum; box-vertebrae ( A , E ). Symbols represent individual larvae (18–64/group) from 3–4 independent experiments. ( I-K ) Larvae were treated as in ( A ) with the indicated retinoid or DMSO and porphyrin fluorescence ( I ), porphyrin excretion ( J ) and operculum volume ( K ) were assayed. Bone volume was normalized to DMSO-treated larvae set to 100%, ( D , H , K ). Porphyrin excretion was normalized to DMSO-treated larvae set to 100%, ( C , G , J ). Symbols represent individual larvae (7–44/group) from 2–4 independent experiments. Scale bars: 200 µm. Three-dimensional image reconstruction ( A , E ) was performed using Imaris 3D software v7.7 ( http://imaris.oxinst.com/ ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Scientific Reports

Article Title: Acitretin mitigates uroporphyrin-induced bone defects in congenital erythropoietic porphyria models

doi: 10.1038/s41598-021-88668-9

Figure Lengend Snippet: Acitretin mitigates CEP bone phenotype in zebrafish. ( A ) 6dpf larvae were injected with uro-I and transferred to medium containing acitretin or DMSO. At 7dpf larvae were injected with calcein and imaged by confocal microscopy. Quantification of porphyrin fluorescence ( B ), porphyrin excretion ( C ) and operculum volume ( D ) from experiment in ( A ). Symbols represent individual larvae (12–25/group) from 3–4 independent experiments. ( E ) 6dpf larvae were injected with uro-I. At 7dpf they were transferred to medium containing acitretin or DMSO. At 8dpf larvae were injected with calcein and imaged by confocal microscopy. Quantification of porphyrin fluorescence ( F ), porphyrin excretion ( G ) and operculum volume ( H ) from experiment in ( E ). Arrowhead-operculum; box-vertebrae ( A , E ). Symbols represent individual larvae (18–64/group) from 3–4 independent experiments. ( I-K ) Larvae were treated as in ( A ) with the indicated retinoid or DMSO and porphyrin fluorescence ( I ), porphyrin excretion ( J ) and operculum volume ( K ) were assayed. Bone volume was normalized to DMSO-treated larvae set to 100%, ( D , H , K ). Porphyrin excretion was normalized to DMSO-treated larvae set to 100%, ( C , G , J ). Symbols represent individual larvae (7–44/group) from 2–4 independent experiments. Scale bars: 200 µm. Three-dimensional image reconstruction ( A , E ) was performed using Imaris 3D software v7.7 ( http://imaris.oxinst.com/ ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: A dose-response curve with acitretin (Selleck Chemicals, Houston, TX) was conducted (0.5–12.5 μM) and 10 μM was observed to yield consistent results, without being toxic to zebrafish larvae.

Techniques: Injection, Confocal Microscopy, Fluorescence, Software

Saos-2 cells mimic CEP zebrafish model. ( A, B ) Mineralization in Saos-2 cells treated with MAC ± Uro-I was assayed using ARS staining (photograph, A; quantification, B). Staining was normalized to MAC only-treated cells (set to 100%). ( C ) Cell lysates from experiment in ( A ) were blotted with the indicated antibodies. ( D ) Quantification of LC3-II shown in (C). LC3-II level was normalized to MAC only (left panel) or vehicle-treated (right panel), set to 100%. ( E ) RT 2 Profiler PCR Array (left panel) and qPCR (right panel). Relative gene expression is represented as fold change normalized to housekeeping gene. Data are from 2 independent experiments. ( F ) Acitretin does not rescue reduced mineral matrix phenotype in uro-I-treated cells. ARS staining quantification as in ( B ). ( G ) Acitretin normalizes ER stress (BiP) and autophagy (LC3-II) markers. Dashed lines represent non-adjacent lanes in the gel. Coomassie-stained gel (C,G) shows equal protein loading. Full-length blots and gels (C,G) are presented in Fig.S4. ( H ) Quantification of LC3-II. LC3-II level was normalized to DMSO-treated cells set to 100%. ( I ) Gene expression profiling as in (E). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Scientific Reports

Article Title: Acitretin mitigates uroporphyrin-induced bone defects in congenital erythropoietic porphyria models

doi: 10.1038/s41598-021-88668-9

Figure Lengend Snippet: Saos-2 cells mimic CEP zebrafish model. ( A, B ) Mineralization in Saos-2 cells treated with MAC ± Uro-I was assayed using ARS staining (photograph, A; quantification, B). Staining was normalized to MAC only-treated cells (set to 100%). ( C ) Cell lysates from experiment in ( A ) were blotted with the indicated antibodies. ( D ) Quantification of LC3-II shown in (C). LC3-II level was normalized to MAC only (left panel) or vehicle-treated (right panel), set to 100%. ( E ) RT 2 Profiler PCR Array (left panel) and qPCR (right panel). Relative gene expression is represented as fold change normalized to housekeeping gene. Data are from 2 independent experiments. ( F ) Acitretin does not rescue reduced mineral matrix phenotype in uro-I-treated cells. ARS staining quantification as in ( B ). ( G ) Acitretin normalizes ER stress (BiP) and autophagy (LC3-II) markers. Dashed lines represent non-adjacent lanes in the gel. Coomassie-stained gel (C,G) shows equal protein loading. Full-length blots and gels (C,G) are presented in Fig.S4. ( H ) Quantification of LC3-II. LC3-II level was normalized to DMSO-treated cells set to 100%. ( I ) Gene expression profiling as in (E). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: A dose-response curve with acitretin (Selleck Chemicals, Houston, TX) was conducted (0.5–12.5 μM) and 10 μM was observed to yield consistent results, without being toxic to zebrafish larvae.

Techniques: Staining, Gene Expression

Proposed model of CEP pathogenesis. UROS inhibition leads to production of uro/copro-I mostly in erythrocytes and liver, which is transported through blood to the bones. Uro-I causes bone damage by binding to hydroxyapatite, causing oxidative and ER stress, protein aggregation and stalled autophagy. Acitretin partially rescues uro-I-induced bone damage by reducing oxidative and ER stress and restoring autophagic flux.

Journal: Scientific Reports

Article Title: Acitretin mitigates uroporphyrin-induced bone defects in congenital erythropoietic porphyria models

doi: 10.1038/s41598-021-88668-9

Figure Lengend Snippet: Proposed model of CEP pathogenesis. UROS inhibition leads to production of uro/copro-I mostly in erythrocytes and liver, which is transported through blood to the bones. Uro-I causes bone damage by binding to hydroxyapatite, causing oxidative and ER stress, protein aggregation and stalled autophagy. Acitretin partially rescues uro-I-induced bone damage by reducing oxidative and ER stress and restoring autophagic flux.

Article Snippet: A dose-response curve with acitretin (Selleck Chemicals, Houston, TX) was conducted (0.5–12.5 μM) and 10 μM was observed to yield consistent results, without being toxic to zebrafish larvae.

Techniques: Inhibition, Binding Assay